Development of antigen detection ELISA for the diagnosis of brugian and bancroftian filariasis using antibodies to recombinant filarial antigens Bm-SXP-1 and Wb-SXP-1.
Identifieur interne : 009475 ( Main/Exploration ); précédent : 009474; suivant : 009476Development of antigen detection ELISA for the diagnosis of brugian and bancroftian filariasis using antibodies to recombinant filarial antigens Bm-SXP-1 and Wb-SXP-1.
Auteurs : Pattabhiraman Lalitha [Inde] ; Devarajan Eswaran ; Munirathinam Gnanasekar ; Kakuturu Venkata Nagaraja Rao ; Rangarajan Badri Narayanan ; Alan Scott ; Thomas Nutman ; Perumal Kaliraj ; Muniratnam GnanasekarSource :
- Microbiology and immunology [ 0385-5600 ] ; 2002.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (immunologie), Anticorps monoclonaux (immunologie), Antigènes d'helminthe (immunologie), Brugia malayi (immunologie), Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Humains, Lapins, Mâle, Protéines d'helminthes (immunologie), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Test ELISA (), Wuchereria bancrofti (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Protéines d'helminthes, Protéines recombinantes, Wuchereria bancrofti.
- Animaux, Humains, Lapins, Mâle, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antibodies, Monoclonal (immunology), Antigens, Helminth (immunology), Brugia malayi (immunology), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay (methods), Helminth Proteins (immunology), Humans, Male, Mice, Mice, Inbred BALB C, Rabbits, Recombinant Proteins (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- immunology : Brugia malayi, Elephantiasis, Filarial, Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Humans, Male, Mice, Mice, Inbred BALB C, Rabbits.
Abstract
Antibodies specific to recombinant filarial antigens Wb-SXP-1 and Bm-SXP-1 have been used to develop a sandwich ELISA for the detection of circulating filarial antigen (CFA) in sera from patients with lymphatic filariasis caused by Wuchereria bancrofti of Brugia malayi. In patients with W. bancrofti infections, a high proportion of microfilaria (mf) positive (MF) and low proportions of patients with chronic pathology (CP) and endemic normals (EN) showed the presence of CFA. Similarly in patients with brugian infections a high proportion of mf positive individuals contained CFA while none of the patients with chronic pathology or endemic normals showed the presence of CFA. Sera from patients with other parasitic infections (OPI) like O. volvulus, Loa loa, Ascaris lumbricoides and from individuals residing in areas non-endemic to filariasis did not exhibit any reactivity. This assay shows promise for the detection of microfilaremic infections in lymphatic filariasis and its usefulness as a diagnostic tool especially in B. malayi infections, needs to be further evaluated.
PubMed: 12139392
Affiliations:
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last="Gnanasekar">Munirathinam Gnanasekar</name></author><author><name sortKey="Rao, Kakuturu Venkata Nagaraja" sort="Rao, Kakuturu Venkata Nagaraja" uniqKey="Rao K" first="Kakuturu Venkata Nagaraja" last="Rao">Kakuturu Venkata Nagaraja Rao</name></author><author><name sortKey="Narayanan, Rangarajan Badri" sort="Narayanan, Rangarajan Badri" uniqKey="Narayanan R" first="Rangarajan Badri" last="Narayanan">Rangarajan Badri Narayanan</name></author><author><name sortKey="Scott, Alan" sort="Scott, Alan" uniqKey="Scott A" first="Alan" last="Scott">Alan Scott</name></author><author><name sortKey="Nutman, Thomas" sort="Nutman, Thomas" uniqKey="Nutman T" first="Thomas" last="Nutman">Thomas Nutman</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author><author><name sortKey="Gnanasekar, Muniratnam" sort="Gnanasekar, Muniratnam" uniqKey="Gnanasekar M" first="Muniratnam" 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sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name></author><author><name sortKey="Rao, Kakuturu Venkata Nagaraja" sort="Rao, Kakuturu Venkata Nagaraja" uniqKey="Rao K" first="Kakuturu Venkata Nagaraja" last="Rao">Kakuturu Venkata Nagaraja Rao</name></author><author><name sortKey="Narayanan, Rangarajan Badri" sort="Narayanan, Rangarajan Badri" uniqKey="Narayanan R" first="Rangarajan Badri" last="Narayanan">Rangarajan Badri Narayanan</name></author><author><name sortKey="Scott, Alan" sort="Scott, Alan" uniqKey="Scott A" first="Alan" last="Scott">Alan Scott</name></author><author><name sortKey="Nutman, Thomas" sort="Nutman, Thomas" uniqKey="Nutman T" first="Thomas" last="Nutman">Thomas Nutman</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author><author><name sortKey="Gnanasekar, Muniratnam" sort="Gnanasekar, Muniratnam" uniqKey="Gnanasekar M" first="Muniratnam" last="Gnanasekar">Muniratnam Gnanasekar</name></author></analytic><series><title level="j">Microbiology and immunology</title><idno type="ISSN">0385-5600</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Rabbits</term><term>Recombinant Proteins (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (immunologie)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Humains</term><term>Lapins</term><term>Mâle</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA ()</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" 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xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Lapins</term><term>Mâle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antibodies specific to recombinant filarial antigens Wb-SXP-1 and Bm-SXP-1 have been used to develop a sandwich ELISA for the detection of circulating filarial antigen (CFA) in sera from patients with lymphatic filariasis caused by Wuchereria bancrofti of Brugia malayi. In patients with W. bancrofti infections, a high proportion of microfilaria (mf) positive (MF) and low proportions of patients with chronic pathology (CP) and endemic normals (EN) showed the presence of CFA. Similarly in patients with brugian infections a high proportion of mf positive individuals contained CFA while none of the patients with chronic pathology or endemic normals showed the presence of CFA. Sera from patients with other parasitic infections (OPI) like O. volvulus, Loa loa, Ascaris lumbricoides and from individuals residing in areas non-endemic to filariasis did not exhibit any reactivity. This assay shows promise for the detection of microfilaremic infections in lymphatic filariasis and its usefulness as a diagnostic tool especially in B. malayi infections, needs to be further evaluated.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Eswaran, Devarajan" sort="Eswaran, Devarajan" uniqKey="Eswaran D" first="Devarajan" last="Eswaran">Devarajan Eswaran</name><name sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name><name sortKey="Gnanasekar, Muniratnam" sort="Gnanasekar, Muniratnam" uniqKey="Gnanasekar M" first="Muniratnam" last="Gnanasekar">Muniratnam Gnanasekar</name><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Narayanan, Rangarajan Badri" sort="Narayanan, Rangarajan Badri" uniqKey="Narayanan R" first="Rangarajan Badri" last="Narayanan">Rangarajan Badri Narayanan</name><name sortKey="Nutman, Thomas" sort="Nutman, Thomas" uniqKey="Nutman T" first="Thomas" last="Nutman">Thomas Nutman</name><name sortKey="Rao, Kakuturu Venkata Nagaraja" sort="Rao, Kakuturu Venkata Nagaraja" uniqKey="Rao K" first="Kakuturu Venkata Nagaraja" last="Rao">Kakuturu Venkata Nagaraja Rao</name><name sortKey="Scott, Alan" sort="Scott, Alan" uniqKey="Scott A" first="Alan" last="Scott">Alan Scott</name></noCountry><country name="Inde"><noRegion><name sortKey="Lalitha, Pattabhiraman" sort="Lalitha, Pattabhiraman" uniqKey="Lalitha P" first="Pattabhiraman" last="Lalitha">Pattabhiraman Lalitha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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